Localization of radiolabeled anti-DNA monoclonal antibodies in murine systemic lupus erythematosus (SLE)
The diagnosis of SLE can be extremely difficult. This multi-system disease is characterized by the deposition of DNA-anti-DNA antibody (Ab) complexes in many tissues, producing glomerulonephritis and systemic vasculitis. This study evaluates an IGG monoclonal (Mo) Ab directe3d against DNA (MrSSl) for potential radioimmunodiagnosis of SLE. Six 15 wk. old F-1 female hybrids of NZB+NZW mice (an animal SLE model that develops vasculitis and nephritis) were injected with 50 ..mu..Cl of I-131 MrSSl and 15 ..mu..Cl of I-125 isotype-matched control mouse myeloma (LPC-1) (non-reactive with DNA). Imaging and tissue distribution were studied. Two animals were also imaged using I-131 LPC Ab. Images at 2 and 9 days showed no clear differences in scan patterns using MrSSl or LPC-1 Ab. Tissue distribution studies at six days, however, showed a significantly higher accumulation of MrSSl in the kidneys vs. control Ab (2.7% vs. 1.8% of injected dose) (p < .04). Similarly, higher levels of MrSS were also seen in the spleen, liver and lungs (p < .03). Blood levels tended to be higher with the specific antibody as well. These differences were not apparent at 3 days post injection. The increased concentration of MrSSl present at 9 days in several organs may be secondary to MrSSl binding to DNA containing immune complexes present in diseased tissues. Blocked clearance by immune complexes or DNA, or differences in electrical charges of the antibodies could be contributing to the higher MrSSl levels seen. Images did not suggest deiodination as responsible. Further studies are necessary to determine if the amount of MrSSl retained by diseased animals is indicative of SLE disease activity.
- Research Organization:
- Univ. of Michigan Medical Center, Ann Arbor, MI
- OSTI ID:
- 6844923
- Report Number(s):
- CONF-840619-; TRN: 87-008764
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 25:5; Conference: 31. annual meeting of the Society of Nuclear Medicine, Los Angeles, CA, USA, 5 Jun 1984
- Country of Publication:
- United States
- Language:
- English
Similar Records
Alteration of the spontaneous systemic autoimmune disease in (NZB x NZW)F1 mice by treatment with thimerosal (ethyl mercury)
Immunopathogenicity and oncogenicity of murine leukemia viruses. II. Infection of mice and rats with Scripps leukemia virus
Related Subjects
IODINE 125
DIAGNOSTIC USES
IODINE 131
LUPUS
DIAGNOSIS
MONOCLONAL ANTIBODIES
LABELLING
ANIMAL TISSUES
BIOLOGICAL ACCUMULATION
CLEARANCE
DNA
IMAGE PROCESSING
INJECTION
LIVER
LUNGS
MICE
NEPHRITIS
SPLEEN
TISSUE DISTRIBUTION
TRACER TECHNIQUES
ANIMALS
ANTIBODIES
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
INFECTIOUS DISEASES
INTAKE
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROCESSING
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SKIN DISEASES
TISSUES
TUBERCULOSIS
UROGENITAL SYSTEM DISEASES
USES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics