Analysis of lung injury in humans by bronchoalveolar lavage after exposure to 0. 60 ppm nitrogen dioxide

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6839984

Smeglin, A M; Utell, M J; Finkelstein, J; Mavis, R; Roberts, Jr, N J; Morrow, P E

Animal studies have shown the utility of bronchoalveolar lavage in investigating responses of the lung to inhaled toxins. To investigate low-level nitrogen dioxide (NO/sub 2/) inhalation in humans, 6 healthy, asymptomatic, non-smoking volunteers were exposed to air or 0.60 ppm NO/sub 2/ by double-blind randomization for 3 hours in a 45 m/sup 3/ environmental chamber. Subjects exercised for 10 minutes every 30 minutes on a bicycle ergometer at a workload sufficient to quadruple minute ventilation. Pulmonary function parameters including FVC, FEV/sub 1/, and airway resistance did not change during or after exposure to NO/sub 2/. 3 1/2 hours after exposure subjects underwent bronchoalveolar lavage. No differences in lavage return volume, total cell recovery, cell viability, or differential cell counts were observed after NO/sub 2/ exposure. Recovery of total protein (14.31 +/- 2.08 (SE) vs 14.72 +/- 1.73 mg) was unchanged. After NO/sub 2/ there was a modest but significant increase in total recovery of aryl sulfatase (964 +/- 188 vs 825 +/- 186 units, 0.01 < p < 0.02) suggesting possible cell injury. Other enzymes including lactate dehydrogenase and malate dehydrogenase did not change. Their results indicate that bronchoalveolar lavage is a sensitive technique for evaluating early injury induced by inhaled toxicants in humans.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 6839984
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LUNGS
INJURIES
NITROGEN DIOXIDE
TOXICITY
INHALATION
LAVAGE
OXIDOREDUCTASES
BODY
CHALCOGENIDES
ENZYMES
INTAKE
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANS
OXIDES
OXYGEN COMPOUNDS
RESPIRATORY SYSTEM
560300* - Chemicals Metabolism &amp; Toxicology