Cellulosome: a discrete cell surface organelle of Clostridium thermocellum which exhibits separate antigenic, cellulose-binding and various cellulolytic activities
A cellulose-binding, cellulase-containing factor, previously demonstrated to be responsible for the adherence of Clostridium thermocellum to cellulose, has been partly purified from cellulose-grown cells of this organism. The biochemical properties of the cell-associated factor were compared to those of the previously isolated extracellular factor, and a high degree of similarity was found in the properties and behavior of the two forms. Partial denaturation of the purified extracellular factor by treatment with sodium dodecyl sulfate at 25/sup 0/C, broke the complex into a reproducible pattern of smaller subcomplexes which were analyzed for their respective cellulolytic activities and corresponding subunit composition. The data indicate that a defined arrangement of endo- and exo-cellulases are organized in the parent complex. The term cellulosome is proposed for the cell-associated, cellulose-binding, multicellulase complex. 20 references, 8 figures, 2 tables.
- Research Organization:
- Tel Aviv Univ., Ramat Aviv, Israel
- OSTI ID:
- 6838299
- Report Number(s):
- CONF-830567-
- Journal Information:
- Biotechnol. Bioeng. Symp.; (United States), Vol. 13; Conference: 5. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN, USA, 10 May 1983
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ENZYMES
CELLULOLYTIC ACTIVITY
CHEMICAL ANALYSIS
ANTIBODIES
ANTIGENS
CELLULASE
CELLULOSE
CHROMATOGRAPHY
CLOSTRIDIUM THERMOCELLUM
COMPARATIVE EVALUATIONS
ELECTROPHORESIS
IN VITRO
MOLECULAR STRUCTURE
MUTANTS
SAMPLE PREPARATION
TRANSMISSION ELECTRON MICROSCOPY
BACTERIA
CARBOHYDRATES
CLOSTRIDIUM
ELECTRON MICROSCOPY
ENZYME ACTIVITY
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
MICROSCOPY
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
SEPARATION PROCESSES
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