Three-dimensional structure of interleukin 8 in solution

Journal Article · Tue Feb 20 00:00:00 EST 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00459a004· OSTI ID:6828854

Clore, G M; Appella, E; Gronenborn, A M ^[1]; Yamada, Masaki ^[2]; Matsushima, Kouji ^[3]

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1. National Institutes of Health, Bethesda, MD (USA)
2. National Cancer Institute, Frederick, MD (USA)
3. Dainippon Pharmaceutical Company, Osaka (Japan)

The solution structure of the interleukin 8 (IL-8) dimer has been solved by nuclear magnetic resonance (NMR) spectroscopy and hybrid distance geometry-dynamical simulated annealing calculations. The structure determination is based on a total of 1,880 experimental distance restraints (of which 82 are intersubunit) and 362 torsion angle restraints (comprising {phi}, {psi}, and {chi}{sub 1} torsion angles). A total of 30 simulated annealing structures were calculated, and the atomic rms distribution about the mean coordinate positions (excluding residues 1-5 of each subunit) is 0.41 {plus minus} 0.08 {angstrom} for the backbone atoms and 0.90 {plus minus} 0.08 {angstrom} for all atoms. The three-dimensional solution structure of the IL-8 dimer reveals a structural motif in which two symmetry-related antiparallel {alpha}-helices, approximately 24 {angstrom} long and separated by about 14 {angstrom}, lie on top of six-stranded antiparallel {beta}-sheet platform derived from two three-stranded Greek keys, one from each monomer unit. The general architecture is similar to that of the {alpha}1/{alpha}2 domains of the human class I histocompatibility antigen HLA-A2. It is suggested that the two {alpha}-helices form the binding site for the cellular receptor and that the specificity of IL-8, as well as that of a number of related proteins involved in cell-specific chemotaxis, mediation of cell growth, and the inflammatory response, is achieved by the distinct distribution of charged and polar residues at the surface of the helices.

• OSTI ID: 6828854
• Journal Information: Biochemistry; (USA), Vol. 29:7; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
IMMUNITY
BIOCHEMISTRY
LYMPHOKINES
MOLECULAR STRUCTURE
GEOMETRY
INFLAMMATION
NUCLEAR MAGNETIC RESONANCE
PROTONS
STEREOCHEMISTRY
BARYONS
CHEMISTRY
ELEMENTARY PARTICLES
FERMIONS
GROWTH FACTORS
HADRONS
MAGNETIC RESONANCE
MATHEMATICS
MITOGENS
NUCLEONS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PROTEINS
RESONANCE
SYMPTOMS
550201* - Biochemistry- Tracer Techniques