The human estrogen receptor can regulate exogenous but not endogenous vitellogenin gene promoters in a Xenopus cell line

Journal Article · Mon Sep 12 00:00:00 EDT 1988 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/16.17.8291· OSTI ID:6810736

Seiler-Tuyns, A; Merillat, A M; Haefliger, D N; Wahli, W ^[1]

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1. Universite de Lausanne (Switzerland)

Transfection of a human estrogen receptor cDNA expression vector (HEO) into cultured Xenopus kidney cells confers estrogen responsiveness to the recipient cells as demonstrated by the hormone dependent expression of co-transfected Xenopus vitellogenin-CAT chimeric genes. The estrogen stimulation of these vit-CAT genes is dependent upon the presence of the vitellogenin estrogen responsive element (ERE) in their 5{prime} flanking region. Thus, functional human estrogen receptor (hER) can be synthesized in heterologous lower vertebrate cells and can act as a trans-acting regulatory factor that is necessary, together with estradiol, for the induction of the vit-CAT constructs in these cells. In addition, vitellogenin minigenes co-transfected with the HEO expression vector also respond to hormonal stimulation. Their induction is not higher than that of the vit-CAT chimeric genes. It suggests that in the Xenopus kidney cell line B 3.2, the structural parts of the vitellogenin minigenes do not play a role in the induction process. Furthermore, no stabilizing effect of estrogen on vitellogenin mRNA is observed in these cells.

• OSTI ID: 6810736
• Journal Information: Nucleic Acids Research; (UK), Vol. 16:17; ISSN 0305-1048
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ESTROGENS
RECEPTORS
GENE REPRESSORS
DNA SEQUENCING
PROTEINS
GENE REGULATION
AMPHIBIANS
CELL CULTURES
CELL DIFFERENTIATION
KIDNEYS
MAN
MESSENGER-RNA
RECOMBINANT DNA
ANIMALS
AQUATIC ORGANISMS
BODY
DNA
HORMONES
MAMMALS
MEMBRANE PROTEINS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
RNA
STEROID HORMONES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry