Tenax-GC extraction technique for residual polychlorinated biphenyl and polyaromatic hydrocarbon analysis in biodegradation assays

Journal Article · Tue Jan 01 00:00:00 EST 1980 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6809333

Shiaris, M P; Sherrill, T W; Sayler, G S

A rapid Tenax-GC extraction technique has been evaluated for use in conjunction with aqueous biodegradation assays for polyaromatic hydrocarbons and polychlorinated biphenyls. The method was quantitatively efficient and reproducible for phenanthrene, but variable and not quantitative for Aroclor 1254 (polychlorinated biphenyls). Aqueous sample volumes and varying concentrations of organic matter influenced polychlorinated biphenyl and polyaromatic hydrocarbon extraction efficiency. Phenanthrene recovery was decreased by soil extract but unaffected by spent bacteriological culture medium. Both types of organic matter cused significant reduction of Aroclor 1254 recovery. Polyaromatic hydrocarbon and polychlorinated biphenyl biodegradation assays, performed with reservoir samples, supported the laboratory evaluation. The study demonstrated the utility of the Tenax-GC extraction technique for phenanthrene analysis in biodegradation assessment; however, Tenax-GC extraction was not appropriate for Aroclor 1254 biodegradation studies.

• Research Organization: Univ. of Tennessee, Knoxville
• OSTI ID: 6809333
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 39:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
AROMATICS
BIODEGRADATION
EXTRACTION
ORGANIC CHLORINE COMPOUNDS
PHENANTHRENE
POLYCYCLIC AROMATIC HYDROCARBONS
QUANTITATIVE CHEMICAL ANALYSIS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CONDENSED AROMATICS
DECOMPOSITION
HYDROCARBONS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
SEPARATION PROCESSES
550200* - Biochemistry
400102 - Chemical & Spectral Procedures
550700 - Microbiology