DNA BP CALLING SW. Automated DNA Base Pair Calling Algorithm
- Ames Laboratory, IA (United States)
The procedure solves the problem of calling the DNA base pair sequence from two channel electropherogram separations in an automated fashion. The core of the program involves a peak picking algorithm based upon first, second, and third derivative spectra for each electropherogram channel, signal levels as a function of time, peak spacing, base pair signal to noise sequence patterns, frequency vs ratio of the two channel histograms, and confidence levels generated during the run. The ratios of the two channels at peak centers can be used to accurately and reproducibly determine the base pair sequence. A further enhancement is a novel Gaussian deconvolution used to determine the peak heights used in generating the ratio.
- Research Organization:
- Iowa State Univ. of Science and Technology, Ames, IA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- OSTI ID:
- 680236
- Report Number(s):
- ESTSC-001312IBMPC00
- Resource Relation:
- Other Information: PBD: 12 Jun 1999
- Country of Publication:
- United States
- Language:
- English
Similar Records
A microchannel electrophoresis DNA sequencing system
A microchannel electrophoresis DNA sequencing system