mRNA-induced expression of the cardiac Na/sup +/-Ca/sup 2 +/ exchanger in Xenopus oocytes
Xenopus oocytes were injected with total mRNA isolated from hearts of 1-day-old chicks. After 5 days of incubation the follicular cell layers were removed and the oocytes were loaded with Na+ by incubation in hypertonic EGTA solution at 37/sup 0/C. The Na+-loaded oocytes accumulated /sup 45/Ca/sup 2 +/ from a Na+-free medium at a 3-18-fold higher rate than noninjected oocytes or oocytes injected with control solution containing no mRNA. Oocytes not subjected to the Na+-loading procedure showed no mRNA-dependent /sup 45/Ca/sup 2 +/ uptake. Size fractionation of the mRNA using sucrose density gradient centrifugation under denaturing conditions led to the identification of a 25 S fraction competent for induction of the Na/sup +/-Ca/sup 2 +/ exchange system.
- Research Organization:
- Univ. of Bern (Switzerland)
- OSTI ID:
- 6801231
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 263:29
- Country of Publication:
- United States
- Language:
- English
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CALCIUM COMPOUNDS
MEMBRANE TRANSPORT
CALCIUM 45
CATIONS
CELL MEMBRANES
CENTRIFUGATION
FROGS
HEART
MESSENGER-RNA
OOCYTES
SODIUM COMPOUNDS
TRACER TECHNIQUES
ALKALI METAL COMPOUNDS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM ISOTOPES
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GERM CELLS
INTERMEDIATE MASS NUCLEI
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ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANES
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
RNA
SEPARATION PROCESSES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques