Hydrodynamic orientation of sperm heads for flow cytometry
Journal Article
·
· Biophys. J.; (United States)
Two specially shaped sample injection tubes orient flat-shaped mature sperm heads in a flow cytometer. Orientation allows control of a photometric difficulty experienced with some flow cytometers in the measurement of fluorescent and scattered light from sperm and other flat cells. Both relative DNA-stain content and a measure of cell flatness can now be determined simultaneously for each cell at the high rates possible with flow instruments.
- Research Organization:
- Lawrence Livermore Lab., CA
- OSTI ID:
- 6790061
- Journal Information:
- Biophys. J.; (United States), Vol. 23:1
- Country of Publication:
- United States
- Language:
- English
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