Distribution of leukocytes labeled with In-111 oxine in dogs with acute inflammatory lesions
The biodistributions of In-111 oxine (with and without leukocyte labeling) of Ga-67 citrate and of In-111 chloride were compared in 30 dogs with chemical and bacterial abscesses and acute joint inflammation. Serial blood samples were taken and tissues radioassayed at 24 hr. The concentration of In-111-oxine leukocytes in all three types of inflammatory lesion was invariably much higher than that of Ga-67 injected simultaneously. For bacterial abscesses, the mean abscess-to-muscle concentration ratio was 3000 for labeled leukocytes and 72 for Ga-67. Aqueous buffered In-111 oxine sulfate solution appeared better for labeling leukocytes than In-111 oxine in ethanol. When In-111 oxine was not incubated with leukocytes before injection, or if the cells were poorly labeled or damaged, the abscess localization was often inferior to that of gallium. Localization of In-111 chloride also appeared inferior to that of gallium. No significant difference in distribution in the major organs or inflammatory lesions was demonstrable between labeled suspensions of pure neutrophils harvested by elutriation and mixed cell suspensions of leukocytes after erythrocyte sedimentation with hydroxyethyl starch. For both types of leukocyte suspension labeled with In-111 oxine, the average recovery of cell-bound activity in the circulating blood at 4 hr was 32% of the administered activity, inferior to that of DFP-32. It is concluded, therefore, that In-111 oxine is a more effective agent than Ga-67 for the detection of acute focal inflammatory lesions if leukocytes are properly labeled, but current techniques are unsatisfactory for the study of neutrophil kinetics.
- Research Organization:
- State Univ. of New York, Syracuse
- OSTI ID:
- 6786229
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 21:11; Conference: 28. annual meeting of the Society of Nuclear Medicine, Las Vegas, NV, USA, 16 Jun 1981
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ABSCESSES
DIAGNOSIS
BONE JOINTS
SCINTISCANNING
INDIUM 111
DIAGNOSTIC USES
BLOOD CIRCULATION
CITRATES
COMPARATIVE EVALUATIONS
DOGS
GALLIUM 67
GAMMA CAMERAS
LEUKOCYTES
MUSCLES
OXINE
QUANTITY RATIO
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CAMERAS
CARBOXYLIC ACID SALTS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
ELECTRON CAPTURE RADIOISOTOPES
GALLIUM ISOTOPES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDIUM ISOTOPES
INFLAMMATION
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PYRIDINES
QUINOLINES
RADIOISOTOPE SCANNING
RADIOISOTOPES
SKELETON
USES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
551001 - Physiological Systems- Tracer Techniques