Atrial natriuretic factor-like activity in rat posterior pituitary
The presence of a biologically active peptide: Atrial Natriuretic Factor (ANF) has been demonstrated in rat and human circulation and ANF is considered now as a new hormone. ANF may be involved in body fluid regulation. A very sensitive radioimmunoassay for rat ANF allowed the authors to search for immunoreactive ANF (IR-ANF) in rat posterior pituitary. Serial dilutions of homogenates of rat posterior pituitary showed a good parallelism with a reference curve in a radioimmunoassay system. The IR-ANF was extracted from rat posterior pituitary homogenates by activated Vycor glass beads. The lyophilized extract was purified by HPLC on C/sub 18/ ..mu.. Bondapak column. The HPLC yielded two IR-ANF peaks. Both isolated ANF-like material showed biological activity. The IR-ANF eluted with 33% acetonitrile, inhibited ACTH-stimulated aldosterone secretion with a similar potency as synthetic (Arg 101 - Tyr 126) ANF (0.7 x 10/sup -10/M). A much less potent ANF-like material was found in the second peak eluted with 36% acetonitrile. They conclude that ANF-like material is present in rat posterior pituitary and this suggest a possible role in ANF on AVP secretion directly in situ.
- Research Organization:
- Clinical Research Institute of Montreal, Quebec
- OSTI ID:
- 6763798
- Report Number(s):
- CONF-8604222-; TRN: 87-010350
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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ACETONITRILE
HOMOGENATES
IMMUNE REACTIONS
LIQUID COLUMN CHROMATOGRAPHY
PITUITARY GLAND
RATS
ADRENAL HORMONES
ALDEHYDES
ANIMALS
BODY
CHROMATOGRAPHY
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ENDOCRINE GLANDS
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HORMONES
HYDROXY COMPOUNDS
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IMMUNOLOGY
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ORGANIC NITROGEN COMPOUNDS
ORGANS
PREGNANES
PROTEINS
RADIOASSAY
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STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
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550201* - Biochemistry- Tracer Techniques