Critical role of extracellular calcium in vanadate-induced renal vasoconstriction

Journal Article · Thu Mar 01 00:00:00 EST 1984 · Am. J. Physiol.; (United States)

OSTI ID:6748168

Benabe, J E; Cruz-Soto, M A; Martinez-Maldonado, M

Intra-arterial infusion of vanadate (VO/sub 4/) in dogs produces a reduction in renal blood flow (RBF), glomerular filtration rate (GFR), urine flow (V), and the fractional excretion of sodium (FE/sub Na/sup +//). To evaluate the role of Ca/sup 2 +/ in these changes VO/sub 4/ was infused into the renal artery in the presence of the calcium antagonists trifluoperazine (TFP), verapamil, or EGTA. TFP inhibited the effect of VO/sub 4/ on RBF (TFP + VO/sub 4/: 64.1, VO/sub 4/: 38.5 ml/min; P < 0.05), GFR (TFP + VO/sub 4/: 22.9, VO/sub 4/: 9.3, ml/min; P < 0.05) and V (TFP + VO/sub 4/: 0.80, VO/sub 4/: 0.38 ml/min; P < 0.05) without changing FE/sub Na/sup +// (TFP + VO/sub 4/: 3.8, VO/sub 4/: 3.2%). Similar changes were obtained with verapamil as well as with EGTA. Furthermore thyroparathyroidectomy (TPTX) decreased serum calcium (control: 8.78, TPTX: 4.98 mg/100ml; P < 0.05) and blunted the effects of VO/sub 4/ on renal hemodynamics. Reestablishing normal serum Ca/sup 2 +/ by an intra-arterial infusion of CaCl/sub 2/ elicited the VO/sub 4/ effects of vasoconstriction and decreased GFR; V was not affected and FE/sub Na/sup +// rose. The data support the idea that influx of extracellular calcium into smooth muscle cells mediates the hemodynamic effects of VO/sub 4/ in the dog. 34 references.

• Research Organization: Univ. of Puerto Rico School of Medicine, San Juan
• OSTI ID: 6748168
• Journal Information: Am. J. Physiol.; (United States), Vol. 246:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ATP
INHIBITION
CALCIUM
BIOCHEMICAL REACTION KINETICS
KIDNEYS
VASOCONSTRICTION
VANADATES
BIOLOGICAL EFFECTS
BLOOD FLOW
DOGS
MUSCLES
POTASSIUM
RESPONSE MODIFYING FACTORS
SODIUM
ALKALI METALS
ALKALINE EARTH METALS
ANIMALS
BODY
ELEMENTS
KINETICS
MAMMALS
METALS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
REACTION KINETICS
TRANSITION ELEMENT COMPOUNDS
VANADIUM COMPOUNDS
VERTEBRATES
560305* - Chemicals Metabolism &amp; Toxicology- Vertebrates- (-1987)