Bone marrow transplantation across major histocompatibility barriers in mice: II. T cell requirement for engraftment in total lymphoid irradiation-conditioned recipients

Journal Article · Mon Mar 01 00:00:00 EST 1982 · Transplantation; (United States)

DOI:https://doi.org/10.1097/00007890-198203000-00007· OSTI ID:6722362

Vallera, D A; Soderling, C C.B.; Carlson, G J; Kersey, J H

Studies were undertaken to examine the role of T lymphocytes in engraftment of bone marrow (BM) in animals conditioned with total lymphoid irradiation (TLI) prior to transplantation across major histocompatability barriers.Donor BM (added as a source of lymphohematopoietic stem cells) and spleen cells (added as a source of graft-versus-host disease (GVHD)-causing cells) were pretreated in vitro with monoclonal anti-Thy-1.2 plus complement (C). T cell-depleted grafts were then given to allogeneic mice conditioned with 900 rad of single dose TLI plus cyclophosphamide (CY). These mice did not engraft. Even in the absence of added spleen cells, elimination of the small T cell population from donor BM grafts prevented engraftment compared with animals that received the same conditioning regimen and untreated donor cells. These control animals demonstrated uniform evidence of engraftment about 1 month after transplantation. Similar findings were reported when recipients were conditioned with fractionated 17 x 100-rad TLI. In TLI plus CY-conditioned recipients, it was also observed that increasing the donation of treated bone marrow cells still did not result in significant engraftment. In contrast to TLI conditioning, when Thy-1.2 plus C-treated donor cells were given to recipients conditioned with total body irradiation (TBI), a high percentage of engraftment was demonstrated by an H-2 microcytotoxicity assay. Plausible mechanisms for these findings are discussed. (JMT)

• Research Organization: Mayo Memorial Building, Minneapolis, MN
• OSTI ID: 6722362
• Journal Information: Transplantation; (United States), Vol. 33:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BONE MARROW
BIOLOGICAL RADIATION EFFECTS
GRAFTS
IMMUNOSUPPRESSION
RADIOINDUCTION
ENDOXAN
FRACTIONATED IRRADIATION
GRAFT-HOST REACTION
LYMPH NODES
LYMPHOCYTES
MICE
MONOCLONAL ANTIBODIES
PARTIAL BODY IRRADIATION
RADIATION CHIMERAS
SKIN
SPLEEN CELLS
X RADIATION
ALKYLATING AGENTS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHIMERAS
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
HEMATOPOIETIC SYSTEM
IMMUNOSUPPRESSIVE DRUGS
IONIZING RADIATIONS
IRRADIATION
LEUKOCYTES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
MOSAICISM
ORGANS
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
TISSUES
TRANSPLANTS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
551000 - Physiological Systems