sup 15 N NMR study on cyanide (C sup 15 N sup minus ) complex of cytochrome P-450 sub cam. Effects of d-camphor and putidaredoxin on the iron-ligand structure

Journal Article · Mon Nov 27 00:00:00 EST 1989 · Journal of the American Chemical Society; (USA)

DOI:https://doi.org/10.1021/ja00202a007· OSTI ID:6719831

Shiro, Yoshitsugu; Iizuka, Tetsutaro ^[1]; Makino, Ryu; Ishimura, Yuzuru ^[2]; Morishima, Isao ^[3]

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1. Institute of Physical and Chemical Research, Saitama (Japan)
2. Keio Univ., Tokyo (Japan)
3. Kyoto Univ. (Japan)

The cyanide (C{sup 15}N{sup {minus}}) complex of Pseudomonas putida cytochrome P-450 (P-450{sub cam}) exhibited well-resolved and hyperfine-shifted {sup 15}N NMR resonances arising from the iron-bound C{sup 15}N{sup {minus}} at 423 and 500 ppm in the absence and presence of the substrate, d-camphor, respectively. The values were smaller than those for cyanide complexes of myoglobin and hemoglobin ({approx} 1000 ppm) but fell into the same range as those for the cyanide complexes of peroxidases ({approx} 500 ppm). The {sup 15}N shift values of P-450{sub cam} were not incompatible with the existence of anionic ligand, such as cysteinyl thiolate anion, at the fifth coordination site of heme iron. The difference in the {sup 15}N chemical shift values between camphor-free and bound enzymes was inferred by the increase in the steric constraint to the Fe-C-N bond upon substrate binding.

• OSTI ID: 6719831
• Journal Information: Journal of the American Chemical Society; (USA), Vol. 111:20; ISSN 0002-7863
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CYANIDES
NMR SPECTRA
CYTOCHROMES
CALCULATION METHODS
CAMPHOR
DATA ANALYSIS
EXPERIMENTAL DATA
IRON
LIGANDS
MEASURING INSTRUMENTS
MEASURING METHODS
DATA
ELEMENTS
INFORMATION
KETONES
METALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PIGMENTS
PROTEINS
SPECTRA
TERPENES
TRANSITION ELEMENTS
550200* - Biochemistry
400200 - Inorganic
Organic
& Physical Chemistry