Neuropeptide K is present in human cerebrospinal fluid

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90386-6· OSTI ID:6699324

Toresson, G; de las Carreras, C; Brodin, E; Bertilsson, L ^[1]

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1. Karolinska Institute, Stockholm (Sweden)

Neurokinin A-like immunoreactivity (NKA-LI) in human cerebrospinal fluid (CSF) was determined by radioimmuno assay (RIA) combined with high performance liquid chromatography (HPLC). The major immunoreactive component did not coelute with NKA, but coeluted with neuropeptide K (NPK), which contains the NKA sequence in its C-terminus. Trypsin treatment of this component from human CSF and of synthetic NPK, produced a substance which coeluted with NKA in the HPLC system. When the NKA-LI was oxidized with hydrogen peroxide and rechromatographed, the immunoreactivity coeluted with NPK sulfoxide. The results indicate that the main part of the NKA-LI in CSF is identical with NPK. The mean concentration of NPK measured in CSF from 6 healthy subjects by HPLC-RIA was 23 + 11 (SD) pmol/L.

• OSTI ID: 6699324
• Journal Information: Life Sciences; (USA), Vol. 46:23; ISSN 0024-3205
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
PEPTIDES
RADIOIMMUNOASSAY
AMINO ACID SEQUENCE
CEREBROSPINAL FLUID
HYDROGEN PEROXIDE
IMMUNE REACTIONS
LIQUID COLUMN CHROMATOGRAPHY
MAN
TRYPSIN
ANIMALS
BIOASSAY
BIOLOGICAL MATERIALS
BODY FLUIDS
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HYDROGEN COMPOUNDS
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IMMUNOASSAY
IMMUNOLOGY
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MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HYDROLASES
PEROXIDES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
SEPARATION PROCESSES
SERINE PROTEINASES
TRACER TECHNIQUES
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550201* - Biochemistry- Tracer Techniques