Low-energy helium-neon laser irradiation increases the motility of cultured human keratinocytes
- Univ. of California, Davis (USA)
Helium-neon (HeNe) laser irradiation is known to stimulate wound healing. We investigated whether the biostimulatory effects of HeNe irradiation result from enhancement of keratinocyte proliferation or motility. HeNe effects on keratinocyte motility were evaluated by irradiating a wounded culture with 0.8 J/cm2 3 times over a 20-h period. At 20 h post-irradiation, videocinemicroscopy and sequential quantitative measurements of the leading edge were taken over a 6-h period. There was a significant difference in migration of the leading edge in irradiated wounds compared to non-irradiated wounded controls (12.0 microns/h vs 4.0 microns/h, p less than 0.0001). To determine if the increase in migration observed in irradiated cultures resulted from a proliferative effect of HeNe irradiation, subconfluent human keratinocyte cultures were irradiated with single or multiple doses of different fluences of HeNe irradiation (0.4 to 7.2 J/cm2) and evaluated 72 h post-irradiation. Irradiated and non-irradiated keratinocyte cultures grown on a microporous membrane surface were co-cultured with irradiated and non-irradiated fibroblasts to determine if HeNe irradiation induced a paracrine effect on keratinocyte proliferation. No significant increase in keratinocyte proliferation was demonstrated in any of these treatments. The biostimulatory effects of HeNe irradiation may now be extended to include enhancement of keratinocyte motility in vitro; this may contribute to the efficacy of HeNe irradiation in wound healing.
- OSTI ID:
- 6693041
- Journal Information:
- Journal of Investigative Dermatology; (USA), Vol. 94:6; ISSN 0022-202X
- Country of Publication:
- United States
- Language:
- English
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ANIMAL CELLS
CELL PROLIFERATION
LASER RADIATION
BIOLOGICAL EFFECTS
WOUNDS
HEALING
CELL CULTURES
HELIUM-NEON LASERS
KERATIN
MAN
ANIMALS
BIOLOGICAL RECOVERY
ELECTROMAGNETIC RADIATION
GAS LASERS
INJURIES
LASERS
MAMMALS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIATIONS
RECOVERY
SCLEROPROTEINS
VERTEBRATES
560400* - Other Environmental Pollutant Effects