Development of a culture medium for large-scale production of cellulolytic enzymes by Trichoderma reesei

Journal Article · Thu Dec 01 00:00:00 EST 1983 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260251216· OSTI ID:6683796

Warzywoda, M; Ferre, V; Pourquie, J

Culture filtrates of CL-847 strain of Trichoderma reesei grown on different carbon sources have been compared. The highest enzyme production is obtained with Whatman CC 41 cellulose: 17.9 mg/ml of soluble proteins and 13.7 units of filter paper (FP) activity. Wood pulps gave lower production values and more viscous culture media. About one-third of maximal enzyme production is obtained on lactose as the sole carbon source. Addition of 0.5% cellulosic inducer to 6% lactose media enhances enzyme production up to the following levels: 14.1 mg/ml of soluble proteins and 9.4 units of FP activity. (Refs. 9).

• Research Organization: Inst. Francais du Petrole, France
• OSTI ID: 6683796
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 25:12
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
CELLULASE
BIOSYNTHESIS
TRICHODERMA VIRIDE
BATCH CULTURE
CELLULOLYTIC ACTIVITY
CELLULOSE
CULTURE MEDIA
LACTOSE
SLURRIES
WOOD
CARBOHYDRATES
DISACCHARIDES
DISPERSIONS
ENZYME ACTIVITY
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
MIXTURES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SUSPENSIONS
SYNTHESIS
TRICHODERMA
550200* - Biochemistry
550700 - Microbiology
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