Genetic-physical mapping of a photosynthetic gene cluster from R. capsulata
Transposon mutagenesis and complementation analysis of the photosynthesis genes in Rhodopseudomonas capsulata is presented utilizing Tn5.7 mutagenized R-primes. The R-prime pRPS404 contains many of the genes necessary for the differentiation of the photosynthetic apparatus. Utilizing homologous recombination, 30 independent copies of Tn5.7 were inserted into the R. capsulata chromosome with subsequent deletion of wild-type alleles. Mutants were characterized by absorption spectroscopy, SDS-PAGE, and determination of capability for photosynthetic growth. Many mutations in the bacteriochlorophyll and carotenoid biosynthetic pathways were isolated. A regulator mutation was isolated affecting reaction-center synthesis as well as a 44 kd heme-containing polypeptide. Complementation analysis using various pRPR404::Tn5.7 plasmids has led to the postulation of transcriptional units. 32 references, 7 figures, 2 tables.
- Research Organization:
- Lawrence Berkeley Lab., CA
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 6654999
- Journal Information:
- Cell; (United States), Vol. 37
- Country of Publication:
- United States
- Language:
- English
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Tn5. 7 construction and physical mapping of pRPS404 containing photosynthetic genes from Rhodopseudomonas capsulata
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Related Subjects
PHOTOSYNTHETIC REACTION CENTERS
GENE MUTATIONS
RHODOPSEUDOMONAS
GENETIC MAPPING
ABSORPTION SPECTROSCOPY
ELECTROPHORESIS
EXPERIMENTAL DATA
GENE RECOMBINATION
GENES
PHOTOSYNTHESIS
PLASMIDS
TRANSCRIPTION
BACTERIA
CELL CONSTITUENTS
CHEMICAL REACTIONS
DATA
INFORMATION
MAPPING
MICROORGANISMS
MUTATIONS
NUMERICAL DATA
PHOTOCHEMICAL REACTIONS
SPECTROSCOPY
SYNTHESIS
550400* - Genetics