Hydrolysis of lignocelluloses by penicillium funiculosum cellulase
Enzymatic hydrolysis of cellulose is a promising method for the conversion of waste cellulose to glucose. During the past few years, the development of this technology has proceeded rapidly, with significant advances made in enzyme production, pretreatment, and hydrolysis. A variety of fungi are reported to produce cellulases but among these Trichoderma reesei and its mutants are powerful producers of cellulases. However, the search for new and possibly better sources of cellulase is continued due to the low levels of beta-glucosidase of T. reesei. Penicillium funiculosum produces a complete cellulase having endo-beta-1,4-glucanase (15-20 U/mL), exo-beta-1,4-glucanase (1.5-2.0 U/mL), and high beta-glucosidase (8-10 U/mL). The saccharification of alkali-treated cotton and bagasse by P. funiculosum enzyme was 70 and 63%, respectively. It was possible to obtain glucose concentration as high as 30% using 50% bagasse. It is of interest that the percent saccharification of cellulosic substrates with the Penicillium enzyme is comparable to that of T. reesei cellulase when the same amount of filter paper activity is used, although the endo-glucanase activity of the latter is two to three times higher. This communication reports the studies on saccharification of lignocelluloses by P. funiculosum cellulase and certain studies on the kinetic aspects. (Refs. 15).
- Research Organization:
- Biochemistry Div, National Chemical Lab, Poona 411 008, India
- OSTI ID:
- 6630614
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 26:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELLULASE
ENZYME ACTIVITY
CELLULOSE
ENZYMATIC HYDROLYSIS
BAGASSE
BIOCHEMICAL REACTION KINETICS
COTTON
GLUCOSE
LIGNIN
PENICILLIUM
SACCHARIFICATION
AGRICULTURAL WASTES
ALDEHYDES
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
KINETICS
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANIC WASTES
PLANTS
POLYSACCHARIDES
REACTION KINETICS
SACCHARIDES
SOLVOLYSIS
WASTES
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