Identification of the cells involved in auxin transport in maize mesocotyl
- Univ. of North Carolina, Chapel Hill (USA)
A study was undertaken to identify by a direct method the cells involved in auxin transport through maize mesocotyl tissue. The auxin photoaffinity labeling agent, 7-({sup 3}H), 5-azidoindole 3-acetic acid (N{sub 3}IAA), was loaded into excised stem tissue from a cut end. Polar transport of this analog was demonstrated over 4 hours by comparing uptake into tissue loaded with N{sub 3}IAA from the apical vs. the basal end. Triiodobenzoic acid, an auxin transport inhibitor, inhibited N{sub 3}IAA uptake into tissue. Tissue which had taken up the photoaffinity labeling agent was photolyzed to covalently fix the radioisotope within cells. This tissue was sectioned and subjected to in situ autoradiography. The outermost cell of epidermal tissue and certain files of cells in vascular tissue were densely labeled indicating that on a per cell basis these two cell types are most actively transporting auxin.
- OSTI ID:
- 6610809
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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