Autotransplantation of hepatic granulomas into the skin of mice with Schistosoma mansoni infection

Journal Article · Wed Sep 01 00:00:00 EDT 1982 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12500045· OSTI ID:6578693

Nishimura, M; Epstein, W L; Fukuyama, K

Hepatic egg granulomas of mice infected with Schistosoma mansoni were transplanted into the skin of the same animal and changes occurring to macrophages, eosinophils, and mast cells over time were studied by light and electron microscopy and by autoradiographic techniques. Disappearance of cellular components about the egg granulomas occurred within 1 week; the entire implant became encapsulated by inflammatory cells and stroma. By 3 weeks mononuclear cells and macrophages reorganized the granulomas around the eggs and neutrophils disappeared. Activated macrophages contained both secretory rough endoplasmic reticulum and lysosomal-dense bodies. Granuloma size increased up to 5 weeks after implantation and mast cells and eosinophils tended to migrate into the granulomas. The mast cell index always remained lower than in the original hepatic granulomas, while eosinophils were seen in large numbers. During 3 to 8 weeks after implantation mononuclear cells undergoing DNA synthesis in the granulomas ranged from 2.9-4.8%. Some 3-week-old autotransplants were injected with /sup 3/H-thymidine and biopsied from 1 to 21 days later. Labeled mononuclear cells peaked in the granulomas by 10 days (24%) and the numbers fell off sharply after that. These findings indicate that autologously implanted schistosome egg granulomas can be maintained successfully in the skin for prolonged periods with marked ingress of macrophages and eosinophils. The autoradiographic data suggest the lesions are high turnover granulomas.

• Research Organization: Department of Dermatology, University of California, San Francisco, USA
• OSTI ID: 6578693
• Journal Information: J. Invest. Dermatol.; (United States), Vol. 79:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EOSINOPHILS
DYNAMIC FUNCTION STUDIES
GRANULOMAS
IMMUNE REACTIONS
MACROPHAGES
MAST CELLS
TRITIUM COMPOUNDS
ISOTOPE APPLICATIONS
AUTORADIOGRAPHY
LIVER
MICE
SCHISTOSOMA
SKIN
TRANSPLANTS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DISEASES
GLANDS
HELMINTHS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
NEOPLASMS
ORGANS
PHAGOCYTES
PLATYHELMINTHS
RODENTS
SOMATIC CELLS
TREMATODES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques
550301 - Cytology- Tracer Techniques
550901 - Pathology- Tracer Techniques