Radiometric measurement of differential metabolism of fatty acid by mycobacteria
An assay system has been developed based on automated radiometric quantification of /sup 14/CO2 produced through oxidation of (1-/sup 14/C) fatty acids by mycobacteria. Two stains of M. tuberculosis (H37Rv and Erdman) and one of M. bovis (BCG) in 7H9 medium (ADC) with 1.0 microCi of one of the fatty acids (butyric, hexanoic, octanoic, decanoic, lauric, myristic, palmitic, stearic, oleic, linoleic and linolenic) were studied. Results previously published on M. lepraemurium (Hawaiian) were also included for comparison. Both strains of M. tuberculosis had maximum /sup 14/CO2 production from hexanoic acid. Oxidation of butyric and avid oxidation of lauric acids were also found with the H37Rv strain but not with Erdman. In contrast, /sup 14/CO2 production by M. bovis was greatest from lauric and somewhat less from decanoic acid. M. lepraemurium showed increasing oxidation rates from myristic, decanoic and lauric acids. Assimilation studies of M. tuberculosis H37Rv confirmed that most of the oxidized substrates were converted into by-products with no change in those from which no oxidation was found. These data suggest that the radiometric measurement of differential fatty acid metabolism may provide a basis of strain identification of the genus Mycobacterium.
- Research Organization:
- Division of Nuclear Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland
- OSTI ID:
- 6569926
- Journal Information:
- Int. J. Lepr.; (United States), Vol. 50:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBOXYLIC ACIDS
METABOLISM
MYCOBACTERIUM TUBERCULOSIS
BUTYRIC ACID
CARBON 14 COMPOUNDS
CARBON DIOXIDE
DECANOIC ACID
DODECANOIC ACID
HEXADECANOIC ACID
HEXANOIC ACID
LINOLEIC ACID
LINOLENIC ACID
OCTADECANOIC ACID
OCTANOIC ACID
OLEIC ACID
RADIOMETRIC ANALYSIS
TETRADECANOIC ACID
TRACER TECHNIQUES
BACTERIA
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
CHEMICAL ANALYSIS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MYCOBACTERIUM
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
550501* - Metabolism- Tracer Techniques
550701 - Microbiology- Tracer Techniques