Anti-idiotypic antisera in man. I. Production and immunochemical characterization of anti-idiotypic antisera to human antitetanus antibodies. [/sup 125/I tracer technique, rabbits]

Journal Article · Sun Oct 01 00:00:00 EDT 1978 · J. Immunol.; (United States)

OSTI ID:6541026

Geha, R S; Weinberg, R P

Antisera were raised in rabbits against immunosorbent-purified F(ab')/sub 2/ fragments of IgG antitetanus toxoid (TT) antibodies obtained from three different donors. The antisera were rendered idiotype specific by absorption with insolubilized TT-nontreactive F(ab')/sub 2/. The resulting anti-idiotypic antisera precipitated more than 85% of /sup 125/I-radiolabeled F(ab')/sub 2/ anti-TT and were shown to be individual specific in that they reacted only with the immunizing F(ab')/sub 2/ anti-TT and not with F(ab')/sub 2/ anti-TT derived from other donors. Anti-idiotypic antiserum inhibited binding of /sup 125/I-TT but not of /sup 125/I-DT to IgG derived from the donor of the immunizing F(ab')/sub 2/ anti-TT, but did not inhibit /sup 125/I-TT binding to IgG derived from other donors. Finally, binding of /sup 125/I-F(ab')/sub 2/ anti-TT to the anti-idiotypic antiserum was completely inhibited by IgG derived from the same donor but only partially inhibited by a great excess of TT antigen, suggesting that the anti-idiotypic antiserum is recognizing nonantigen-binding idiotypic determinants on F(ab')/sub 2/ anti-TT. The data presented demonstrate that anti-idiotypic heteroantisera can be successfully raised against human antibodies to TT, indicate minimal cross-reactivity of idiotypic determinants between unrelated individuals, and suggest the presence of nonantigen binding-idiotypic determinants on the antibody molecule.

• Research Organization: Children's Hospital Medical Center, Boston
• OSTI ID: 6541026
• Journal Information: J. Immunol.; (United States), Vol. 121:R4
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
IMMUNE SERUMS
RADIOIMMUNOASSAY
TETANUS
IMMUNE REACTIONS
ANTIBODIES
IMMUNOGLOBULINS
IODINE 125
RABBITS
TOXOIDS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOISOTOPES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics