Elution of benzo(a)pyrene from carbon blacks into biomembranes in vitro
Elution of benzo(a)pyrene (BaP) from carbon blacks into phospholipid vesicles composed of dimyristoyl phosphatidylcholine (DMPC) or dipalmitoyl phosphatidylcholine (DPPC) was investigated. Samples of rubber-grade carbon blacks designated N-234, N-339, N-351, and N-375 containing exogenously adsorbed BaP were prepared by solvent extracting endogenous compounds from the carbon blacks and then readsorbing BaP at the desired concentrations. Concentrations of exogenous BaP on the carbon blacks were approximately 100-fold higher than normally occur on commercial carbon blacks, but the higher levels were used to improve the ability to detect elution of BaP into membranes. Elution of BaP from carbon blacks was studied using a fluorescence spectroscopic technique. Elution from N-234 was below detection limits of the system. Elution of BaP from N-339, N-351, and N-375 into DMPC and DPPC vesicles occurred in a biphasic manner. Elution occurred within 60 min of mixing carbon blacks with vesicles, although not all BaP was eluted from the particles. When the concentration of BaP adsorbed to N-375 was reduced, the rate and extent of elution were lowered. Extent of elution of BaP in these experiments may be greater than that of endogenous BaP on commercial carbon blacks because of the considerably higher quantities of exogenous BaP present. Furthermore, solvent extraction of endogenous materials prior to readsorption of BaP may alter the adsorption characteristics.
- Research Organization:
- Virginia Polytechnic Institute and State Univ., Blacksburg
- OSTI ID:
- 6525886
- Journal Information:
- J. Toxicol. Environ. Health; (United States), Vol. 15:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BENZOPYRENE
BIOLOGICAL AVAILABILITY
LEACHING
CARBON BLACK
FLUORESCENCE SPECTROSCOPY
IN VITRO
MEMBRANES
PHOSPHOLIPIDS
RESPONSE MODIFYING FACTORS
SOLVENT EXTRACTION
AROMATICS
CARBON
CONDENSED AROMATICS
DISSOLUTION
ELEMENTS
EMISSION SPECTROSCOPY
ESTERS
EXTRACTION
HYDROCARBONS
LIPIDS
NONMETALS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
SEPARATION PROCESSES
SPECTROSCOPY
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)