Characterization of liposomes containing iodine-125-labeled radiographic contrast agents
Multilamellar liposomes were prepared containing either iodine-125-labeled (/sup 125/I) diatrizoate or /sup 125/I labeled iotrol in their aqueous phase. The in vitro permeabilities of liposomes containing both contrast agents were measured in the presence of saline and serum at 37 degrees C. Two different phospholipid compositions were studied: phosphatidylcholine/cholesterol/stearylamine (PC/C/S, 8: 1:1 molar ratio) and distearoylphosphatidylcholine/sphingomyelin (DSPC/SM, 5:2 mole ratio). In saline, similar permeabilities were observed for the four phospholipid-contrast agent combinations. In serum, however, leakage of /sup 125/I activity was 2 to 3 times greater from PC/C/S liposomes than from vesicles composed of DSPC/SM. When PC/C/S liposomes that contained /sup 125/I-diatrizoate were injected into rats, the clearance half-times for /sup 125/I activity from the liver, spleen, and whole body were 4.4 hours, 4.5 hours, and 2.8 hours, respectively. Liposomes composed of DSPC/SM cleared at a significantly slower rate from the liver, spleen, and whole body with half-times of 24.0 hours, 18.4 hours, and 17.2 hours observed from these tissues, respectively.
- Research Organization:
- Harvard Medical School, Boston, MA
- OSTI ID:
- 6524061
- Journal Information:
- Invest. Radiol.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Increased delivery of gallium-67 to tumors using serum-stable liposomes
Interactions of /sup 14/N:/sup 15/N stearic acid spin-label pairs: effects of host lipid alkyl chain length and unsaturation
Related Subjects
LIPOSOMES
CHEMICAL COMPOSITION
PERMEABILITY
CLEARANCE
COMPUTERIZED TOMOGRAPHY
CONTRAST MEDIA
IODINE 125
IODINE COMPOUNDS
LABELLED COMPOUNDS
METABOLISM
PHOSPHOLIPIDS
RATS
TISSUE DISTRIBUTION
ANIMALS
BETA DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
HALOGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LIPIDS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
RADIOISOTOPES
RODENTS
TOMOGRAPHY
VERTEBRATES
550301* - Cytology- Tracer Techniques