In vivo transport kinetics and distribution of taurocholate by rat ileum and jejunum
- Burroughs Wellcome Company, Research Triangle Park, NC (USA)
The transport kinetics and distribution of taurocholate (TC) from the ileum and jejunum were evaluated in anesthetized Sprague-Dawley rats. Uptake and transport kinetics were determined after simultaneous administration of equimolar solutions of ({sup 3}H)taurocholic acid (({sup 3}H)TC) in the ileum and (24-{sup 14}C)taurocholic acid (({sup 14}C)TC) in the jejunum of anesthetized rats. At TC concentrations between 0.3 and 15 mM, total ileal absorption exceeded jejunal absorption 15- to 25-fold. The apparent Km and Vmax for ileal absorption of TC were 5.6 mM and 65.5 nmol.min-1.cm-1, respectively. Tissue distribution studies following uptake from the ileum and jejunum were done with {sup 75}Se-labeled homocholic acid taurine ({sup 75}Se-HCAT). In the ileum, 82% of the {sup 75}Se-HCAT removed from the lumen was found in the bile, 10.6% was found in the ileal wall, and 7.3% was found in the liver. In the jejunum, 24.1% was found in bile, 0.6% was found in the liver, and 75.3% remained in the jejunal wall. These data show that the ileum is much more efficient and better equipped than the jejunum to take up and transport TC at concentrations up to 15 mM.
- OSTI ID:
- 6522292
- Journal Information:
- American Journal of Physiology; (USA), Vol. 259:2 Pt 1; ISSN 0002-9513
- Country of Publication:
- United States
- Language:
- English
Similar Records
Taurocholate transport by brush-border membrane vesicles from the developing rabbit ileum: Structure/function relationships
Intestinal sucrase inhibitors and bile acid absorption in the rat
Related Subjects
CHOLIC ACID
MEMBRANE TRANSPORT
CARBON 14 COMPOUNDS
IN VIVO
INTESTINAL ABSORPTION
ISOTOPE DILUTION
RATS
SELENIUM 75
SMALL INTESTINE
TAURINE
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
ABSORPTION
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BILE ACIDS
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RADIOISOTOPES
RODENTS
SELENIUM ISOTOPES
STEROIDS
STEROLS
SULFONIC ACIDS
TRACER TECHNIQUES
UPTAKE
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques