Lithium inhibits terminal differentiation of erythroleukemia cells: evidence for a pre-commitment priming event

Journal Article · Sun Feb 01 00:00:00 EST 1987 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(87)90012-7· OSTI ID:6515859

Zaricznyj, C; Macara, I G

Lithium has been found to be a novel inhibitor of the terminal differentiation of Friend murine erythroleukemia cells. A general method for the quantitative analysis of differentiation inhibitors has been developed and used to compare the site of inhibition by lithium with that by vanadate. Lithium inhibits the commitment to differentiation (K/sub 1/2/approx.10 mM) induced by DMSO (dimethylsulfoxide) at non-toxic concentrations that have only a small effect on the rate of proliferation. Inhibition is reversible and probably requires entry of Li/sup +/ into the cell, since it is blocked by high KCl in the medium. LiCl is most effective when present during the first 10 h of DMSO treatment, before commitment is initiated. Computer-assisted analysis of the kinetics of commitment demonstrate that inhibition by lithium is best described by including a lithium-sensitive priming event, which occurs with high probability prior to commitment.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 6515859
• Journal Information: Exp. Cell Res.; (United States), Vol. 168:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
62 RADIOLOGY AND NUCLEAR MEDICINE
LITHIUM CHLORIDES
BIOLOGICAL EFFECTS
RUBIDIUM 86
UPTAKE
TUMOR CELLS
CELL DIFFERENTIATION
INHIBITION
LEUKEMIA
RUBIDIUM CHLORIDES
VANADATES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHLORIDES
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
DISEASES
HALIDES
HALOGEN COMPOUNDS
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LITHIUM COMPOUNDS
LITHIUM HALIDES
MINUTES LIVING RADIOISOTOPES
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
OXYGEN COMPOUNDS
RADIOISOTOPES
RUBIDIUM COMPOUNDS
RUBIDIUM ISOTOPES
TRANSITION ELEMENT COMPOUNDS
VANADIUM COMPOUNDS
560300* - Chemicals Metabolism & Toxicology
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