Effects of structural features of cotton cellulose on enzymatic hydrolysis. [Gamma radiation]

Journal Article · Mon Jun 01 00:00:00 EDT 1981 · J. Appl. Polym. Sci.; (United States)

DOI:https://doi.org/10.1002/app.1981.070260622· OSTI ID:6507678

Focher, B ^[1]; Cattaneo, M; Beltrame, P L; Carniti, P

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1. Stazione Sperimentale per la Cellulosa, Carta e Fibre Tessili Vegetali ed Artificiali, Milan, Italy

Textile cotton wastes were treated with ..gamma.. rays and 18% NaOH and 70% ZnCl/sub 2/ solutions and were subjected to enzymatic hydrolysis. The untreated and treated samples were characterized both before and after hydrolysis by means of parameters concerning molecular structure (degree of polymerization), supermolecular structure (x-ray diffraction), accessibility, and reactivity (moisture regain, enzyme adsorption, and solubility in FeTNa). These parameters were correlated to kinetic parameters of the hydrolysis reaction. The V/sub max/ and K/sub m/ values were evaluated from Lineweaver-Burk plots at different temperatures. The V/sub max//K/sub m/ ratio, analogous to the specificity constant, proved to be less sensitive to experimental errors and more suitable for a comparison of the kinetic behavior of the samples. The modifications of both supermolecular structure and morphology of cellulose were of primary importance to attain high yields and rates of hydrolysis. Furthermore, the structural and morphologic parameters chosen to characterize the samples can be correlated to the kinetic parameters of enzymatic hydrolysis, in particular to K/sub m/ values.

• OSTI ID: 6507678
• Journal Information: J. Appl. Polym. Sci.; (United States), Vol. 26:6
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
CELLULOSE
CHEMICAL RADIATION EFFECTS
ENZYMATIC HYDROLYSIS
CHEMICAL REACTION KINETICS
ADSORPTION
CELLOBIOSE
CELLULASE
COMPARATIVE EVALUATIONS
COTTON
ENZYMES
GAMMA RADIATION
IRON COMPOUNDS
MORPHOLOGY
POLYMERIZATION
SODIUM COMPOUNDS
SODIUM HYDROXIDES
SOLUBILITY
STRUCTURAL CHEMICAL ANALYSIS
TARTRATES
VISCOSITY
X-RAY DIFFRACTION
ZINC CHLORIDES
ALKALI METAL COMPOUNDS
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CHEMICAL REACTIONS
CHEMISTRY
CHLORIDES
CHLORINE COMPOUNDS
COHERENT SCATTERING
DECOMPOSITION
DIFFRACTION
DISACCHARIDES
ELECTROMAGNETIC RADIATION
GLYCOSYL HYDROLASES
HALIDES
HALOGEN COMPOUNDS
HYDROGEN COMPOUNDS
HYDROLASES
HYDROLYSIS
HYDROXIDES
IONIZING RADIATIONS
KINETICS
LYSIS
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
SACCHARIDES
SCATTERING
SOLVOLYSIS
SORPTION
TRANSITION ELEMENT COMPOUNDS
ZINC COMPOUNDS
ZINC HALIDES
400600* - Radiation Chemistry