Radiation induced gram negative bacteremia and endotoxemia in rabbits: modification by anti-lipopolysaccharide hyperimmune equine plasma

Journal Article · Mon Jun 29 00:00:00 EDT 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90077-4· OSTI ID:6497629

Well, M T; Gaffin, S L; Jordaan, J P

Lethal whole-body irradiation damages the gut mucosa and leads to leakage of endotoxin or lipopolysaccharides (LPS) into the systemic circulation. Sixteen rabbits, irradiated with 900 rads /sup 60/Co, were randomly divided on day 4 into 2 groups, one of which received an intraperitoneal injection of normal saline (control) and the other (experimental) an equal volume of anti-LPS hyperimmune plasma. The time course of endotoxemia and bacteremia were determined for the duration of the experiment. While rabbits in both groups died within 13 days after irradiation, rabbits given saline died on average 2 days earlier, than rabbits given anti-LPS plasma. Plasma LPS concentrations rose to a small peak on day 2 prior to treatment. Thereafter plasma LPS in rabbits given saline increased forty fold by day 9. In contrast, in rabbits given anti-LPS plasma, LPS concentrations in the plasma remained within pretreatment limits. By day 12 after irradiation, plasma anti-LPS IgG had declined to 5.8% of pretreatment levels in rabbits given saline as compared to 46% in rabbits given anti-LPS plasma (p 0.005). While both groups developed gram-positive bacteremia, rabbits given saline in addition also developed gram-negative bacteremia. No rabbits treated with Anti-LPS showed gram-negative bacteremia.

• Research Organization: Univ. of Natal Medical School, Congella, South Africa
• OSTI ID: 6497629
• Journal Information: Life Sci.; (United States), Vol. 40:26
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MUCOUS MEMBRANES
BIOLOGICAL RADIATION EFFECTS
RABBITS
WHOLE-BODY IRRADIATION
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
COBALT 60
INTESTINES
LETHAL IRRADIATION
LIPOPOLYSACCHARIDES
SURVIVAL CURVES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
COBALT ISOTOPES
DIGESTIVE SYSTEM
DRUGS
EXTERNAL IRRADIATION
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIPIDS
MAMMALS
MEMBRANES
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
RADIATION EFFECTS
RADIOISOTOPES
SACCHARIDES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560152* - Radiation Effects on Animals- Animals