In vitro assessment of the cell-mediated immune response to herpes simplex virus in man
These studies demonstrated that human peripheral blood mononuclear cells (PBMC) from individuals sensitized to herpes simplex virus type 1 (HSV-1) were capable of producing interleukin 2 (IL 2) following in vitro stimulation with HSV antigen. IL 2 activity was detected by the direct addition of the murine IL 2-dependent cell line, CTLL-20, to ..gamma..-irradiated cultures of HSV-1 antigen-stimulated PBMC. It was found that PBMC from sensitized individuals produced IL 2 in a dose-dependent manner after in vitro stimulation with HSV antigen. Furthermore, IL 2 production in response to viral antigen correlated with viral antigen-induced proliferation of PBMC. It was also shown that contact with HSV-1 antigen induced the expression of IL 2 receptors on a small percentage of human PBMC. While this suggested that IL 2 receptor expression was associated with viral antigen-induced proliferation responses, the level of induced IL 2 receptor expression remained close to the lower limit of detectability for cytofluorographic analysis. Experiments to elucidate the role of the macrophage (MO) in the response to viral antigen revealed that HSV antigen-induced IL 2 production by sensitized T lymphocytes was dependent on the presence of an accessory MO. To investigate the signals provided to T lymphocytes by accessory cells, MOs were pulsed with HSV antigen and treated with paraformaldehyde. This allowed HSV antigen display, but prevented monokine (IL 1) secretion. The treated MOs could no longer induce sensitized lymphocytes to produce IL 2.
- Research Organization:
- Minnesota Univ., Minneapolis (USA)
- OSTI ID:
- 6472755
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
Similar Records
A distinct population of nonphagocytic and low level CD4+ null lymphocytes produce IFN-alpha after stimulation by herpes simplex virus-infected cells
Antigen-specific immune-suppressor factor in herpes simplex virus type 2 infections of UV B-irradiated mice
Related Subjects
IMMUNOSUPPRESSION
RADIOINDUCTION
LEUKOCYTES
CHEMICAL ACTIVATION
DRUGS
GAMMA RADIATION
HERPES SIMPLEX
IMMUNITY
IN VITRO
RECEPTORS
VIRUSES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DISEASES
ELECTROMAGNETIC RADIATION
INFECTIOUS DISEASES
IONIZING RADIATIONS
MATERIALS
MEMBRANE PROTEINS
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIATIONS
SKIN DISEASES
VIRAL DISEASES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture