Nucleotide sequence of the gene from the cyanobacterium Anacystis nidulans R2 encoding the Mn-stabilizing protein involved in photosystem II water oxidation
The gene for the Mn-stabilizing protein (MSP; the so-called extrinsic 33-kDa protein) that is involved in photosystem II water oxidation was cloned and sequenced from the genome of the cyanobacterium Anacystis nidulans R2. The gene (here designated woxA) was shown to be present in a single copy. The deduced amino acid sequence indicated that the translation product consisted of 277 amino acid residues with a M/sub r/ of 29,306. The comparison of the sequence with that of mature MSP from spinach chloroplasts suggested that the translation product is a precursor whose amino-terminal 28 amino acid residues represent the signal peptide for the protein to cross the thylakoid membrane into the lumen. The length of the putative signal peptide was less than half that of the transit peptide for thylakoid-lumenal proteins of higher plants, whereas the structural profile of the putative signal peptide was similar to that of the carboxyl-terminal portion of the higher plant transit peptide. The amino acid sequence of the mature A. nidulans R2 MSP showed rather low homology (48-49%) to higher plant MSPs, but the conserved amino acid residues appeared to be clustered. Five clusters were tentatively assigned, in which the homology values were in a range of 66-70%. Domains essential for the functioning of MSP are expected to be situated in these clusters. It is of note that the two cysteine residues in MSP were conserved, and the disulfide linkage between them may play an important role in maintaining the tertiary structure of MSP.
- Research Organization:
- Univ. of Missouri, Columbia (USA)
- DOE Contract Number:
- FG02-86ER13516
- OSTI ID:
- 6468551
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:23
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHLOROPHYLL-BINDING PROTEINS
AMINO ACID SEQUENCE
GENES
DNA SEQUENCING
CYANOBACTERIA
DNA-CLONING
HYBRIDIZATION
MANGANESE COMPOUNDS
OXIDATION
PHOTOSYNTHETIC REACTION CENTERS
SPINACH
WATER
CHEMICAL REACTIONS
CLONING
DNA HYBRIDIZATION
FOOD
HYDROGEN COMPOUNDS
MICROORGANISMS
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PLANTS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSITION ELEMENT COMPOUNDS
VEGETABLES
550201* - Biochemistry- Tracer Techniques