Fermentative production of butanol from sorghum molasses as a potential agricultural fuel. Final report, June 26, 1981-September 25, 1982
A strain, Clostridium acetobutylicum ATCC 4259, suitable for butanol-acetone fermentation of sorghum molasses was selected from several strains of the American Type Culture Collection (ATCC). It was cultivated in the composition-optimized sorghum molasses medium. The microbial growth and sugar consumption pattern in the sorghum molasses medium exhibited a typical diauxie phenomenon. The results strongly suggest that the difficulty encountered by the Weizmann type of organisms in butanol-acetone fermentation of molasses is due to the diauxie phenomenon causing a significant decrease in the solvent production rate. Acid hydrolysis of sorghum molasses minimizes the occurrence of the phenomenon, thereby remarkably increasing the solvent yield. The final solvent concentrations in the inverted molasses medium were butanol, 1.0% (w/v); acetone, 0.37% (w/v); ethanol, 0.18% (w/v); and total solvent, 1.55% (w/v). The total solvent yield in the inverted sorghum molasses medium was 30.3% based on the weight of sugar consumed. Effects of the temperature, agitation and heat-shocking were also investigated.
- Research Organization:
- Kansas State Univ., Manhattan (USA). Dept. of Chemical Engineering
- DOE Contract Number:
- FG07-81ID12331
- OSTI ID:
- 6398375
- Report Number(s):
- DOE/ID/12331-T1; ON: DE83008248
- Resource Relation:
- Other Information: Portions are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACETONE
BIOSYNTHESIS
BUTANOLS
CLOSTRIDIUM ACETOBUTYLICUM
GROWTH
MOLASSES
FERMENTATION
ACID HYDROLYSIS
ETHANOL
SOLVENTS
YIELDS
ALCOHOLS
BACTERIA
BIOCONVERSION
CHEMICAL REACTIONS
CLOSTRIDIUM
DECOMPOSITION
HYDROLYSIS
HYDROXY COMPOUNDS
KETONES
LYSIS
METHANOGENIC BACTERIA
MICROORGANISMS
ORGANIC COMPOUNDS
SOLVOLYSIS
SYNTHESIS
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)