Molecular cloning and functional expression in heterologous mammalian cells of the cDNAs encoding lactate dehydrogenase isozymes A (muscle), B (heart), and C (testis) from man and mouse

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6360835

Sakai, I; Hou, E W; Sharief, F S; Hiraoka, B Y; Takano, T; Li, S S.L.

The full-length cDNAs encoding human LHD-A (muscle), human and mouse LDH-B (heart), and mouse LDH-C (testis) isozymes have been isolated and their nucleotide sequences determined. The cDNAs for human LDH-A and mouse LDH-C isozymes were inserted into mammalian expression vectors and were shown to direct the synthesis of functional LDH isozymes in Chinese hamster ovary cells. The exogeneous LDH-A and LDH-C subunits were found to form hybrid LDH tetrameric isozymes with endogeneous LDH-A subunit of CHO cells. The genomic DNA containing LDH cDNA insert and the levels of mRNA expression are being characterized.

• Research Organization: NIEHS, Research Triangle Park, NC
• OSTI ID: 6360835
• Report Number(s): CONF-870644-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ISOENZYMES
AMINO ACID SEQUENCE
DNA-CLONING
OXIDOREDUCTASES
CHO CELLS
HEART
MAN
MESSENGER-RNA
MICE
MUSCLES
TESTES
ANIMAL CELLS
ANIMALS
BODY
CARDIOVASCULAR SYSTEM
CLONING
ENZYMES
GONADS
MALE GENITALS
MAMMALS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
RNA
RODENTS
VERTEBRATES
550200* - Biochemistry