In vivo ultraviolet-exposed human epidermal cells activate T suppressor cell pathways that involve CD4+CD45RA+ suppressor-inducer T cells

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Journal of Immunology; (USA)

OSTI ID:6359969

Baadsgaard, O; Salvo, B; Mannie, A; Dass, B; Fox, D A; Cooper, K D ^[1]

---

1. Univ. of Michigan Medical School, Ann Arbor (USA)

In vivo UV exposure of human epidermis abrogates the function of CD1+DR+ Langerhans cells and induces the appearance of CD1-DR+ Ag-presenting macrophages. Epidermal cells from UV-exposed skin, in contrast to epidermal cells from normal skin, potently activate autologous CD4+ T cells, and, in particular, the CD45RA+ (2H4+) (suppressor-inducer) subset. We therefore determined whether UV-exposure in humans leads to a T cell response in which suppression dominates. Autologous blood T cells were incubated with epidermal cell suspensions from in vivo UV-irradiated skin. After activation, repurified T cells were transferred in graded numbers to autologous mononuclear cells (MNC) stimulated with PWM and the resultant IgG production analyzed by ELISA. Relative to T cells activated by unirradiated control epidermal cells, T cells activated by UV-exposed epidermal cells demonstrated enhanced capacity to suppress IgG production (n = 6; p less than or equal to 0.03). Within the T cell population, CD8+ cells stimulated by UV-exposed epidermal cells could be directly activated to suppress PWM-stimulated MNC Ig production if IL-2 was provided in the reaction mixture. The suppressive activity was also transferable with purified CD4+ T cells stimulated by UV-exposed epidermal cells (n = 10; p less than or equal to 0.01), and was radiosensitive. Suppression was decreased when PWM-stimulated MNC were depleted of CD8+ T cells before mixing with CD4+ T cells activated by UV-exposed epidermal cells, suggesting indirect induction of CD8+ Ts cells contained within the responding MNC populations. Indeed, physical depletion of CD45RA+ cells resulted in total abrogation of the suppressor function contained in the CD4+ T cells. Activation of suppressor function was critically dependent on DR+ APC contained in UV-exposed epidermis.

• OSTI ID: 6359969
• Journal Information: Journal of Immunology; (USA), Vol. 145:9; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
EPIDERMIS
BIOLOGICAL RADIATION EFFECTS
IMMUNOGLOBULINS
BIOSYNTHESIS
LYMPHOCYTES
CELL PROLIFERATION
ANTIBODY FORMATION
ANTIGENS
BIOLOGICAL PATHWAYS
ENZYME IMMUNOASSAY
MAN
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
EPITHELIUM
GLOBULINS
IMMUNOASSAY
LEUKOCYTES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIATION EFFECTS
RADIATIONS
SKIN
SOMATIC CELLS
SYNTHESIS
TISSUES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture