Serum prostate-specific acid phosphatase: development and validation of a specific radioimmunoassay. [/sup 125/I tracer technique]
We describe radioimmunoassay for human prostatic acid phosphatase (orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2) in serum, with use of monospecific antisera raised in rabbits against highly purified acid phosphatase from human prostates. The antiserum did not cross react with partly purified acid phosphatases from human spleen, erythrocytes, or synovial tissues. /sup 125/I-labeled acid phosphatase was prepared by a Chloramine T method, and the bound and free antigen was separated in the assay by use of anti-rabbit gamma-globulin raised in sheep. Uniform low nonspecific binding of the (/sup 125/I)acid phosphatase was achieved by using acid-phosphatase-free serum to prepare standard curves and diluted samples of serum with high acid phosphatase activities. Concentrations of immunoreactive acid phosphatase in the serum of healthy men ranged from <1 to 10 ..mu..g/liter and for 12 patients with advanced prostatic carcinoma between 100 and 500 ..mu..g/liter. The concentrations of the enzyme in sera of patients with benign prostatic hyperplasia were very similar to those in sera of the reference group.
- Research Organization:
- Univ. of Oulu, Finland
- OSTI ID:
- 6340643
- Journal Information:
- Clin. Chem.; (United States), Vol. 24:11
- Country of Publication:
- United States
- Language:
- English
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PROSTATE
BLOOD SERUM
CHLORAMINES
IMMUNE SERUMS
IODINE 125
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TRACER TECHNIQUES
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
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GLANDS
HYDROLASES
INTERMEDIATE MASS NUCLEI
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ORGANIC CHLORINE COMPOUNDS
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ORGANIC HALOGEN COMPOUNDS
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VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics