Simultaneous determination of selenite and trimethylselenonium ions in urine by anion exchange chromatography and molecular neutron activation analysis

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00144a012· OSTI ID:6337017

Blotcky, A J; Hansen, G T; Borkar, N; Ebrahim, A; Rack, E P

A method has been developed for the determination of trimethylselenonium (TMSe) ion and selenite (SeO/sub 3//sup 2 -/) ion in urine by anion exchange chromatography, selectively eluting TMSe and SeO/sub 3//sup 2 -/. Since the recoveries are quantitative, a method of additive spikes is employed to determine TMSe and SeO/sub 3//sup 2 -/ ion concentrations in urine specimens. The TMSe and SeO/sub 3//sup 2 -/ chromatographic elutions were collected in vials, irradiated with neutrons, and radioassayed for /sup 77m/Se activity. The limit of detection is 10 ng of Se as TMSe or SeO/sub 3//sup 2 -//ml of urine. Thirteen urine specimens from normal subjects were analyzed for TMSe, SeO/sub 3//sup 2 -/, and total selenium concentration.

• Research Organization: Veterans Administration Medical Center, Omaha, NE
• DOE Contract Number: FG02-84ER13231
• OSTI ID: 6337017
• Journal Information: Anal. Chem.; (United States), Vol. 59:17
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
ORGANIC COMPOUNDS
ACTIVATION ANALYSIS
ION EXCHANGE CHROMATOGRAPHY
SELENITES
SELENIUM COMPOUNDS
URINE
ANIONS
EXPERIMENTAL DATA
NEUTRON REACTIONS
QUANTITATIVE CHEMICAL ANALYSIS
TRACE AMOUNTS
BARYON REACTIONS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHARGED PARTICLES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
HADRON REACTIONS
INFORMATION
IONS
MATERIALS
NUCLEAR REACTIONS
NUCLEON REACTIONS
NUMERICAL DATA
OXYGEN COMPOUNDS
SEPARATION PROCESSES
WASTES
400101* - Activation
Nuclear Reaction
Radiometric & Radiochemical Procedures
550200 - Biochemistry
400105 - Separation Procedures