Differential phosphorylation of multiple sites in protein 4. 1 and protein 4. 9 by phorbol ester-activated and cyclic AMP-dependent protein kinases
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:6335772
The phosphorylation of the membrane skeleton components protein 4.1 and protein 4.9 in intact erythrocytes is shown to increase in the presence of either 1 microM 12-O-tetradecanoyl phorbol 13-acetate or 2 mM dibutyryl cAMP. The phosphorylation induced by these protein kinase activators is compared by two-dimensional tryptic peptide mapping. In both proteins, the pattern of peptides phosphorylated in the presence of 12-O-tetradecanoyl phorbol 13-acetate differs from the pattern of peptides phosphorylated in the presence of dibutyryl cAMP. The relative locations of the phosphorylated sites on protein 4.1 have been determined using limited proteolysis by alpha-chymotrypsin.
- Research Organization:
- Yale Univ. School of Medicine, New Haven, CT
- OSTI ID:
- 6335772
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 16
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMP
BIOLOGICAL EFFECTS
PHORBOL ESTERS
PHOSPHORUS 32
TRACER TECHNIQUES
PROTEINS
PHOSPHORYLATION
ERYTHROCYTES
KINETICS
PHOSPHOTRANSFERASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MATERIALS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
RADIOISOTOPES
TRANSFERASES
550201* - Biochemistry- Tracer Techniques
AMP
BIOLOGICAL EFFECTS
PHORBOL ESTERS
PHOSPHORUS 32
TRACER TECHNIQUES
PROTEINS
PHOSPHORYLATION
ERYTHROCYTES
KINETICS
PHOSPHOTRANSFERASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MATERIALS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
RADIOISOTOPES
TRANSFERASES
550201* - Biochemistry- Tracer Techniques