(Analysis of proteins essential for Agrobacterium mediated DNA transfer to plant cells). [Single-stranded DNA binding proteins]
The tumor inducing (Ti) plasmid of Agrobacterium contains two regions important for infection and transformation of plant cells. One region, the T-DNA, is transferred as a single strand into the plant cell, while the virulence (vir) region is responsible for recognition of susceptible cells, synthesis of the T-DNA single strand (T-strand), formation of a T-strand protein complex and transfer of this complex into susceptible cells. A DNA binding protein, VirE2, was identified as a product of the vir region. Sequencing of the 9000 kilobase pair virB region has been completed. Expression of 10 of the predicted 11 open reading frames (ORFs) was demonstrated in Escherichia coli. Translational coupling was demonstrated for 5 ORFs. Hydropathy analysis indicates that 9 of 11 ORFs have hydrophobic regions that could permit membrane channel formation. In related work, analysis of protons that potentiate movement of plant viruses was discussed, with indications that the tobacco mosaic virus (TMV) protein P30 may mediate transfer of TMV RNA through plasmadesmata. Also, using the T-DNA element as a marker, genes responsible for abnormal flower development are being cloned and isolated. 3 refs. (MHB)
- Research Organization:
- California Univ., Berkeley, CA (USA). Dept. of Plant Biology
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- FG03-88ER13882
- OSTI ID:
- 6324031
- Report Number(s):
- DOE/ER/13882-T1; ON: DE91006871
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MEMBRANE PROTEINS
PLANT CELLS
ONCOGENIC TRANSFORMATIONS
PLASMIDS
DNA SEQUENCING
DNA-CLONING
ARABIDOPSIS
BACTERIA
DNA HYBRIDIZATION
FLOWERS
MEMBRANE PORES
MEMBRANE TRANSPORT
PLANT GROWTH
PROGRESS REPORT
RNA
TOBACCO MOSAIC VIRUS
TUMOR CELLS
ANIMAL CELLS
CELL CONSTITUENTS
CELL TRANSFORMATIONS
CLONING
DOCUMENT TYPES
GROWTH
HYBRIDIZATION
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PLANTS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
VIRUSES
550400* - Genetics
550200 - Biochemistry