Liquid chromatographic determination of benzo(a)pyrene in total particulate matter of cigarette smoke

Journal Article · Sun Sep 01 00:00:00 EDT 1985 · J. Assoc. Off. Anal. Chem.; (United States)

OSTI ID:6295365

Tomkins, B A; Jenkins, R A; Griest, W H; Reagan, R R; Holladay, S K

The benzo(a)pyrene (BaP) delivery of reference and commercially available tobacco cigarettes, as well as reference and placebo marijuana cigarettes, is determined using a sequential liquid chromatographic/liquid chromatographic procedure. The total particulate matter of sample cigarette smoke is collected using a Cambridge filter pad, which is ultrasonically extracted with acetone. The resulting extract is filtered, then fractionated using semipreparative-scale normal phase liquid chromatography (LC). Quantitative determination is achieved using analytical-scale reverse phase LC equipped with a fluorescence detector. The method is precise (+/- 10-15% relative standard deviation) and yields 85% or better BaP recovery at the ng/cig. level. A single pad may be analyzed in 8 person-hours, while a more typical lot of 12 pads (6 pads each for 2 cigarette brands) may be analyzed in 10 person-days.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 6295365
• Journal Information: J. Assoc. Off. Anal. Chem.; (United States), Vol. 5
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BENZOPYRENE
LIQUID COLUMN CHROMATOGRAPHY
TOBACCO SMOKES
FRACTIONATION
FLUORESCENCE
MARIHUANA
PARTICULATES
AEROSOLS
AROMATICS
CHROMATOGRAPHY
COLLOIDS
CONDENSED AROMATICS
DISPERSIONS
HERBS
HYDROCARBONS
LUMINESCENCE
ORGANIC COMPOUNDS
PARTICLES
PLANTS
RESIDUES
SEPARATION PROCESSES
SMOKES
SOLS
500200* - Environment
Atmospheric- Chemicals Monitoring & Transport- (-1989)
560306 - Chemicals Metabolism & Toxicology- Man- (-1987)