Vasoactive intestinal polypeptide provokes acetylcholine release from the myenteric plexus
Effects of vasoactive intestinal polypeptide (VIP) on the release of acetylcholine (ACh) from longitudinal muscle strips with myenteric plexus (LM) preparations were examined in the guinea pig small intestine. VIP (10 to 10 W M) induced a concentration-dependent contraction of LM preparation. The VIP-induced contractions seem to be related to three components, the scopolamine-sensitive, the scopolamine-insensitive, the tetrodotoxin-sensitive, and the tetrodotoxin-insensitive contractions. VIP (10 to 10 W M) induced a concentration-dependent increase in the release of (TH)ACh from LM preparations preloaded with (TH)choline. The VIP-evoked (TH)ACh release was inhibited by removal of CaS from the perfusion medium and by treatment with tetrodotoxin but not by scopolamine and hexamethonium. The spontaneous and VIP-evoked (TH)ACh release was not affected by phentolamine, propranolol, methysergide, diphenhydramine, cimetidine, bicuculline, or (D-ProS, D-Trp/sup 7,9/)substance P. The result demonstrates that VIP induces contractions of longitudinal smooth muscle directly and indirectly by the stimulation of both cholinergic neurons and noncholinergic excitatory neurons.
- Research Organization:
- Kobe Univ. School of Medicine, Japan
- OSTI ID:
- 6291772
- Journal Information:
- Am. J. Physiol.; (United States), Vol. 251:1
- Country of Publication:
- United States
- Language:
- English
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ACETYLCHOLINE
SECRETION
POLYPEPTIDES
BIOLOGICAL EFFECTS
TRITIUM COMPOUNDS
CARDIOVASCULAR AGENTS
CHOLINE
DOSE-RESPONSE RELATIONSHIPS
GUINEA PIGS
MUSCLES
SMALL INTESTINE
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
DIGESTIVE SYSTEM
DRUGS
ESTERS
GASTROINTESTINAL TRACT
HYDROXY COMPOUNDS
INTESTINES
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PEPTIDES
PROTEINS
QUATERNARY COMPOUNDS
RODENTS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques