Ozone interaction with rodent lung: effect on sulfhydryls and sulfhydryl-containing enzyme activities

Journal Article · Sat Jan 01 00:00:00 EST 1972 · J. Lab. Clin. Med.; (United States)

OSTI ID:6288862

DeLucia, A J; Hogue, P M; Mustafa, M G; Cross, C E

Exposure of rats or rat lung homogenates to 2 ppM O/sub 3/ reduced total, protein, and non-protein sulfhydryl fractions in a dose-related fashion. The non-protein fraction was more susceptible to O/sub 3/ attack. Chronic O/sub 3/ exposure (0.8 ppM, 10 days) had no effect. Activities of SH-containing enzymes, glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD), in lung tissue decreased 10 to 15 and 30%, respectively, after 6 to 8 h exposure to 2 ppM O/sub 3/. In vitro exposure (2 to 3 ppM) led to reductions of 20 and 67%. Activities of cytochrome C reductases decreased 20% after acute exposure but increased 20% after chronic. Chronic exposure increased SH-generating enzyme, G6PD, activity in lung tissue 32% but had little effect on GR activity. Lipid peroxidation (as possible mechanism for SH oxidation) not affected by in vivo exposure but was increased almost 100% in vitro. G6PD activity signals hexose-monophosphate shunt pathway for NADPH production. This pathway is also active in various other diseases (e.g., toxic liver injury).

• OSTI ID: 6288862
• Journal Information: J. Lab. Clin. Med.; (United States), Vol. 80
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ENZYMES
BIOCHEMICAL REACTION KINETICS
RESPONSE MODIFYING FACTORS
OZONE
BIOLOGICAL EFFECTS
DOSE-RESPONSE RELATIONSHIPS
BIOLOGICAL PATHWAYS
CYTOCHROMES
DEHYDROGENASES
GLUCOSE
GLUTATHIONE
HEXOSES
HOMOGENATES
IN VITRO
IN VIVO
LIPIDS
LUNGS
NADP
OXIDATION
OXIDOREDUCTASES
PEROXIDASES
PROTEINS
RATS
THIOLS
ALDEHYDES
ANIMALS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
COENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
KINETICS
MAMMALS
MONOSACCHARIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDES
PIGMENTS
POLYPEPTIDES
PORPHYRINS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SACCHARIDES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550200 - Biochemistry