UV-induced mutagenesis of phage S13 can occur in the absence of the RecA and UmuC proteins of Escherichia coli

Journal Article · Tue Oct 01 00:00:00 EDT 1985 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.82.19.6614· OSTI ID:6286950

Tessman, I

The UV-induced mutagenesis of phage S13 that accompanies Weigle repair is known to require the products of the recA and umuDC genes, as does the UV-induced mutagenesis of the Escherichia coli chromosome. The author found that UV-induced mutagenesis of phage S13 occurred in the absence of both the RecA and UmuC functions when the irradiated phage was photoreactivated. Furthermore, UV-induced phage mutations were produced in a recA- umuC- cell even without photoreactivation and in the absence of any other known UV repair mechanism, at a frequency 29% of that found after photoreactivation and 7% of that found after Weigle repair, implying that DNA synthesis can proceed past a dimer at an unexpectedly high frequency even when unaided by the UmuC-RecA SOS repair functions. The unaided DNA synthesis appears capable of producing mutations in the vicinity of a pyrimidine dimer; by aiding synthesis past a dimer, a repair mechanism may disclose a mutation without having any active role in producing it.

• Research Organization: Purdue Univ., West Lafayette, IN (United States)
• OSTI ID: 6286950
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 19
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MUTATIONS
RADIOINDUCTION
PYRIMIDINE DIMERS
DNA REPAIR
BACTERIOPHAGES
DNA REPLICATION
ESCHERICHIA COLI
PROTEINS
ULTRAVIOLET RADIATION
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
ELECTROMAGNETIC RADIATION
MICROORGANISMS
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
PARASITES
RADIATIONS
RECOVERY
REPAIR
VIRUSES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)