Quantification of cell death in developing cerebellum by a /sup 14/C tracer method
To study the question of whether or not cell death contributes significantly to normal or stressed postnatal brain development in a way which is biochemically quantifiable, we carried out an experiment to assess the amount of cell death in developing cerebellum. By measuring the loss of DNA content and the loss of /sup 14/C from labelled thymidine previously incorporated into the DNA fraction (DNAF) in X-irradiated neonatal animals, shown by histological methods to have cell death to the degree of degranulating the external granular layer (EGL), we showed that when cells die both label and DNA content are greatly decreased in the cerebellum. Experiments on both normal and malnourished animals showed that cell death does not contribute significantly to cerebellar development in either malnutrition-stressed or normal animals. Here, we present a biochemical tool for assessing cell death and evidence that cell death does not contribute significantly to cerebellar development.
- Research Organization:
- Univ. of Texas, Dallas (USA)
- OSTI ID:
- 6276894
- Journal Information:
- Brain Res. Bull.; (United States), Vol. 3:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
BIOLOGICAL STRESS
CEREBELLUM
CELL DIFFERENTIATION
CARBON 14 COMPOUNDS
DNA
NEONATES
NUTRITION
RATS
SURVIVAL TIME
THYMIDINE
TRACER TECHNIQUES
VIABILITY
X RADIATION
ANIMALS
AZINES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATIONS
RIBOSIDES
RODENTS
VERTEBRATES
550901* - Pathology- Tracer Techniques