Induction and regeneration of autoplasts from Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200
Journal Article
·
· Applied and Environmental Microbiology
OSTI ID:625676
- Univ. of Georgia, Athens, GA (United States)
Autolysis was induced to form stable, cell wall-free cells of Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200, using a complex medium containing glycine (0.4% wt/vol) and/or sucrose or glycerol (10% wt/vol) at an optimum temperature of 64{degrees}C. Autoplasts of both bacteria were grown as L-phase colonies on solid medium; more than 50% of these colonies regenerated to the walled form during prolonged incubation. The removal of the cell wall was confirmed by electron microscopy.
- DOE Contract Number:
- FG09-89ER14059
- OSTI ID:
- 625676
- Journal Information:
- Applied and Environmental Microbiology, Vol. 59, Issue 10; Other Information: PBD: Oct 1993
- Country of Publication:
- United States
- Language:
- English
Similar Records
The hemicellulases from the ethanologenic thermophile: Themoanaerobacter ethanolius
Characterization of a symbiotic coculture of Clostridium thermohydrosulfuricum YM3 and clostridium thermocellum YM4
Comparison of alcohol dehydrogenases from wild-type and mutant strain, JW200 Fe 4, of `Thermoanaerobacter ethanolicus`
Technical Report
·
Wed May 01 00:00:00 EDT 1991
·
OSTI ID:625676
Characterization of a symbiotic coculture of Clostridium thermohydrosulfuricum YM3 and clostridium thermocellum YM4
Journal Article
·
Mon Jan 01 00:00:00 EST 1990
· Applied and Environmental Microbiology; (USA)
·
OSTI ID:625676
Comparison of alcohol dehydrogenases from wild-type and mutant strain, JW200 Fe 4, of `Thermoanaerobacter ethanolicus`
Journal Article
·
Thu Dec 31 00:00:00 EST 1992
· Applied Microbiology and Biotechnology
·
OSTI ID:625676