Differential blood-brain barrier permeabilities to (/sup 14/C)sucrose and (/sup 3/H)inulin after osmotic opening in the rat
The blood-brain barrier (B-BB) in 3-month-old rats was opened unilaterally by infusing 1.8 m L(+)arabinose in water into the internal carotid artery through a catheter in the external carotid. Two poorly penetrating uncharged test radiotracers of differing molecular weight and size, (/sup 14/C)sucrose (340 daltons, radius 5 A) and (/sup 3/H)inulin (5500 daltons, radius 15 A), were simultaneously injected i.v. in untreated rats, or rats at 1, 30, or 50 min after infusion of hypertonic arabinose solution. Evans-blue solution was injected 5 min prior to osmotic treatment as a visual indicator of barrier integrity. In regions of uninfused control brains, the (/sup 14/C)sucrose permeability-surface area (PA) product approximated 10(-5) s-1, whereas PA was not measurable for (/sup 3/H)inulin. In arabinose-infused animals, PA products on the ipsilateral hemisphere for both (/sup 14/C)sucrose and (/sup 3/H)inulin were markedly elevated 6 min after infusion, but decreased by 35 and 55 min. In nearly all regions, statistically significant differences were not found between 6-min (/sup 14/C)sucrose- and (/sup 3/H)inulin-PA values (P greater than 0.05). However, at 35 and 55 min in most regions, the PA for (/sup 3/H)inulin was significantly lower (P less than 0.05) than PA for (/sup 14/C)sucrose. The results indicated that the B-BB closed more rapidly to larger than to smaller molecules after osmotic treatment and were consistent with a pore model for osmotic B-BB opening.
- Research Organization:
- National Institute on Aging, Gerontology Research Center, Baltimore, Maryland
- OSTI ID:
- 6255266
- Journal Information:
- Exp. Neurol.; (United States), Vol. 79:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ARABINOSE
BIOLOGICAL EFFECTS
BLOOD-BRAIN BARRIER
PERMEABILITY
INULIN
SACCHAROSE
CARBON 14 COMPOUNDS
MEMBRANE TRANSPORT
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALDEHYDES
ANIMALS
CARBOHYDRATES
DISACCHARIDES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
POLYSACCHARIDES
RODENTS
SACCHARIDES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques