The effect of peripheral lymphoid cells on the incidence of lethal graft versus host disease following allogeneic mouse bone marrow transplantation
Experiments were performed to study the role of circulating lymphoid cells in the incidence of lethal graft versus host disease (GVHD) in radiation-induced fully allogeneic mouse chimeras. The incidence of GVHD was reduced significantly in BALB/c leads to C57BL/6 radiation chimeras if bone marrow donors were exsanguinated immediately prior to marrow harvest. Chimeras resulting from the injection of bone marrow from bled donors exhibited only donor cells in spleen, bone marrow and peripheral blood and normal levels of Thy 1+ and Ia+ cells were found in each of these lymphoid compartments. The addition of as few as 3 X 10(4) peripheral mononuclear cells to the marrow from exsanguinated donors uniformly led to lethal GVHD. /sup 51/Cr-labeled cell traffic studies revealed that prior exsanguination of marrow donors led to about a 70% reduction in the number of circulating mononuclear cells contaminating the bone marrow at the time of marrow harvest. This decrease in contaminating peripheral cells was calculated to be in the appropriate range to account for the decreased GVHD seen when marrow from exsanguinated donors was used. It thus appears that peripheral cells contaminating marrow can be an important factor in causing lethal GVHD in allogeneic radiation chimeras. These results raise the possibility that the fulminant GVHD seen in human marrow transplantation is in part due to the major contamination of bone marrow with peripheral blood that results from the techniques currently used for human bone marrow harvest.
- Research Organization:
- Surgery Branch, Division of Cancer Treatment, National Cancer Institute, National Institutes of Health, Bethesda, Maryland
- OSTI ID:
- 6219904
- Journal Information:
- J. Surg. Res.; (United States), Vol. 34:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Radiation-induced mouse chimeras: a cellular analysis of the major lymphoid compartments, factors affecting lethal graft versus host disease and host-tumor interactions
Bone marrow transplantation across major histocompatability barriers in mice. III. Treatment of donor grafts with monoclonal antibodies directed against Lyt determinants
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BONE MARROW
TRANSPLANTS
LYMPHOCYTES
BIOLOGICAL FUNCTIONS
MONOCYTES
GRAFT-HOST REACTION
CHROMIUM 51
MICE
PATHOLOGY
RADIATION CHIMERAS
RADIOINDUCTION
TRACER TECHNIQUES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHIMERAS
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FUNCTIONS
HEMATOPOIETIC SYSTEM
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MOSAICISM
NUCLEI
ORGANS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
560151* - Radiation Effects on Animals- Man
550301 - Cytology- Tracer Techniques