Human homologue of murine tumor rejection antigen gp96: 5 prime -Regulatory and coding regions and relationship to stress-induced proteins

Journal Article · Wed Aug 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.15.5658· OSTI ID:6215612

Maki, R G; Srivastava, P K ^[1]; Old, L J ^[2]

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1. City Univ. of New York, NY (USA)
2. Sloan-Kettering Institute for Cancer Research, New York, NY (USA)

Cell-surface glycoproteins of 96 kDa (gp96) have been implicated in immunogenicity of methylcholanthrene-induced mouse sarcomas in syngeneic hosts. In view of the potential immunogenicity of gp96-related molecules in human tumors, the authors have defined the 5{prime}-regulatory and complete coding regions of a human gp96 transcript. The 5{prime}-regulatory region contains an imperfect heat shock element apart from other regulatory sequences. The amino acid sequence of human gp96 is 96% homologous to its murine counterpart and genes for the two molecules show significant homology between untranslated regions. Comparison of gp96 sequences to other sequences in DNA and protein data bases indicates significant homology with the stress proteins 94-kDa glucose-regulated protein (grp94) and 108-kDa heat shock protein (hsp108) and 99-kDa endoplasmic reticular protein (ERp99). These molecules are either identical or represent a family of closely related molecules. With regard to their role in tumor immunity, it needs to be determined whether gp96 molecules are tumor antigens per se or whether they serve as carriers of other immunogenic moieties.

• OSTI ID: 6215612
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:15; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIGENS
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
SARCOMAS
IMMUNOLOGY
3-METHYLCHOLANTHRENE
GENES
GLUCOSE
GLYCOPROTEINS
MAN
TRANSCRIPTION
ALDEHYDES
ANIMALS
AROMATICS
CARBOHYDRATES
DISEASES
DNA
HEXOSES
HYDROCARBONS
MAMMALS
MOLECULAR STRUCTURE
MONOSACCHARIDES
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
PRIMATES
PROTEINS
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry
560300 - Chemicals Metabolism & Toxicology