Expansion of the. alpha. sub 2 -adrenergic receptor family: Cloning and characterization of a human. alpha. sub 2 -adrenergic receptor subtype, the gene for which is located on chromosome 2

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.13.5094· OSTI ID:6214411

Lomasney, J W; Lorenz, W; Allen, L F; King, K; Caron, M G; Lefkowitz, R J ^[1]; Regan, J W ^[2]; Yang-Feng, T L ^[3]

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1. Duke Univ. Medical Center, Durham, NC (USA)
2. Univ. of Arizona, Tucson (USA)
3. Yale Univ. School of Medicine, New Haven, CT (USA)

Pharmacologic, biochemical, and genetic analyses have demonstrated the existence of multiple {alpha}{sub 2}-adrenergic receptor ({alpha}{sub 2}AR) subtypes. The authors have cloned a human {alpha}{sub 2}AR by using the polymerase chain reaction with oligonucleotide primers homologous to conserved regions of the previously cloned {alpha}{sub 2}ARs, the genes for which are located on human chromosomes 4 (C4) and 10 (C10). The deduced amino acid sequence encodes a protein of 450 amino acids whose putative topology is similar to that of the family of guanine nucleotide-binding protein-coupled receptors, but whose structure most closely resembles that of the {alpha}{sub 2}ARs. Competition curve analysis of the binding properties of the receptor expressed in COS-7 cells with a variety of adrenergic ligands demonstrates a unique {alpha}{sub 2}AR pharmacology. Hybridization with somatic cell hybrids shows that the gene for this receptor is located on chromosome 2. Northern blot analysis of various rat tissues shows expression in liver and kidney. The unique pharmacology and tissue localization of this receptor suggest that this is an {alpha}{sub 2}AR subtype not previously identified by classical pharmacological or ligand binding approaches.

• OSTI ID: 6214411
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:13; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
HETEROCHROMOSOMES
GENETIC MAPPING
MEMBRANE PROTEINS
AMINO ACID SEQUENCE
RECEPTORS
TISSUE DISTRIBUTION
CATECHOLAMINES
DNA HYBRIDIZATION
KIDNEYS
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PHOSPHORUS 32
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ISOTOPES
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MAMMALS
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550201* - Biochemistry- Tracer Techniques