Release of fermentable carbohydrate from peat by steam explosion and its use in the microbial production of solvents
Steam treatment of peat at 200/sup 0/C for 3 min, followed by instantaneous decompression (steam explosion), solubilized up to 28% of the dry matter. Seventy-five percent of the solubilized material was carbohydrate, 33% of which was composed of mono- and disaccharides, including galactose, glucose, xylose, mannose, arabinose, and cellobiose, in order of decreasing concentration. The solubilized materials served as the sole source of carbohydrate for growth and solvent production by Clostridium acetobutylicum and C. butylicum which utilized up to 40% of the carbohydrate. Of the saccharides in this mixture, galactose was the least readily utilized. Approximately 30% of the fermentable carbohydrate used was converted to fatty acids and solvents, with the primary fermentation product being butyrate. Clostridium thermohydrosulfuricum was able to utilize ca. 50% of the carbohydrate, and simultaneously produced slightly more than 1 mol ethanol/mol saccharide metabolized. This organism, like other strains tested, used galactose less readily than the other sugars. The residue from the steam explosion process contained 24% cellulose, but it could not serve as a source of carbohydrate for the growth of either Bacteroides succinogenes or Clostridium thermocellum, suggesting that inhibitors were released during the steam treatment.
- Research Organization:
- Univ. of Guelph, Ontario
- OSTI ID:
- 6199994
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 28:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum
Declining carbohydrate solubilization with increasing solids loading during fermentation of cellulosic feedstocks by Clostridium thermocellum: documentation and diagnostic tests
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CARBOHYDRATES
FERMENTATION
ETHANOL
YIELDS
PEAT
CHEMICAL COMPOSITION
CHEMICAL FEEDSTOCKS
CLOSTRIDIUM
GROWTH
SOLVENTS
STEAM
ALCOHOLS
BACTERIA
BIOCONVERSION
ENERGY SOURCES
FOSSIL FUELS
FUELS
HYDROXY COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC MATTER
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
550700 - Microbiology