Metabolism analysis and on-line physiological state diagnosis of acetone-butanol fermentation
- Osaka Univ., Suita, Osaka (Japan). International Center of Cooperative Research in Biotechnology
- Prince of Songkla Univ., Bangkok (Thailand). Dept. of Industrial Biotechnology
Fermentation equations for acetone-butanol (AB) were applied in a metabolic analysis of the reaction network under various conditions; that is, at different pHs and a high NADH{sub 2} turnover rate using methyl viologen, in a Clostridium acetobutylicum culture. The results disclosed variations in the pattern of rate changes that reflected changes in the physiological state. A liner relationship was found to exist between NADH{sub 2} generation and butanol production rate. By coupling an automated measurement system with the fermentation model, on-line estimation of the culture state was accomplished. Based on the AB fermentation model, new parameters were defined for on-line diagnosis of the physiological state and determination of the best timing for amplifying NADH{sub 2} generation by the addition of methyl viologen to obtain a high level of butanol productivity. A potential means of achieving optimal control for a high level of solvent production, involving the correlation of certain rates, is proposed.
- OSTI ID:
- 619428
- Journal Information:
- Biotechnology and Bioengineering, Vol. 58, Issue 6; Other Information: PBD: 20 Jun 1998
- Country of Publication:
- United States
- Language:
- English
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